Friday, November 29, 2019

Sugar Plum Bakery

Table of Contents Introduction Allergy Competition Macro Environment Conclusion Reference List Introduction Sugar Plum Bakery (SPB) is a bakery retail business established in Camarillo, California and targets regular and loyal customer with a passion for baked products. The company’s commitments are to provide delicious nut-free baked goods using high-quality ingredients at a reasonable cost to the consumer. Advertising We will write a custom essay sample on Sugar Plum Bakery specifically for you for only $16.05 $11/page Learn More The company’s management has also evaluated and taken into consideration consumers allergic to nuts. To deal with this situation, Sugar Plum Bakery plans to provide delectable homemade treats in a safe environment for consumers allergic to nuts. The company’s goals and objectives are to build a strong and counterproductive market position in Camarillo. This can be achieved by positively benefiting its people in business partnership and improved competitive climate in the area. Allergy Allergy is an abnormal reaction of one’s body after contamination with allergens by inhalation, ingestion, injection or skin contact. There has been much research on food allergy in general but not much on peanut allergy alone. Sugar plum bakery has evolutionary grown with the increased in demand of peanut by consumers globally. Scientist and health professions have defined a substance called allergen to be the primary course of allergy which contains proteins in the food we eat. Peanut allergy is common in children that vary from person to person according to ones immune system. Allergy health professionals have established clinic all over the world to help diagnose allergic patients. In some countries like the US, the Federal Food Allergen and Consumer Protection Act (FALCPA) require that all packaged food must be labeled specifying ingredients contained in the package (Masi, 1995, 78). A research has shown that food allergies are mostly coursed by the sensitivity of the peanut. Sugar Plum Bakery is determined to eliminate allergy problems to consumers at all cost. The cost of our company’s product will be affordable to all consumers with an equalized profit for the company’s management and development. Environmental management in Camarillo is in the interest of Sugar Plum Bakery. Furthermore, the health of Camarillo residents is our main concern in partnership with the local business men. Our products will be freely distributed to local businesses at a minimum cost. Our business is strategically located and readily accessible from all corners of Camarillo with enough space for expansion (Joshi, 2005, 39).Advertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Competition Competitors are business rivals or one who is competing against the other. Sugar Plum Bakery bu siness has been on the increase due to demand in its products i.e. peanut. This has lead to competition between businesses entrepreneurs involved in Sugar Plum Bakery. However, the company has survived the competition and has been able to keep cat bay rigorous competitors. Sugar Plum Bakery is a competitive business in today’s market especially due to high consumption and demand of its product globally. The quality of a company’s product improves its demand by the consumers (Joshi, 2005, 34). A companies’ share do not necessary mean how a company is performing relative to its competitors. Any change in business field like in sales affects market and economic situation. Market share can be defined by the hike in demands and high sale of products leading to competitive profits in comparison with other competitors. Established customer distribution increases share distribution. Product matchup is the comparison of product from other companies for competition. Our company’s product will be of high quality to favor all consumers. Macro Environment A condition that exists in an economy that affects the company planning and performance, and is beyond its control. Business is sources of income that improves economic growth of any given country, city or society. Market is a place where buyers and seller make transaction directly or indirectly. Marketing has evolved over the year where transaction of good to good in old age to today’s computer technology. History has defined exchange of good and product since the creation of the world which intern due to human intelligence has been made easer whereby you can buy product from wherever you maybe just by a click of the button (Lenskold, 2003, 78). Companies all around the world have joined hands to improving the living standard of people. Sugar Plum Bakery products demand has extensively increased due to consumers. Products demand and retail supply, increase in import and marketing eff orts. Supermarket retailer continues to strive for lower labor and capital cost. Advertising We will write a custom essay sample on Sugar Plum Bakery specifically for you for only $16.05 $11/page Learn More Sugar Plum Bakery has large players who have continuously dominated the market but still small retail firms remain active. Small retail exists in the business trying their level best to compete with large volume firms (Keller, 2002, 67). Conclusion Sugar Plum Bakery (SPB) main target is the local people of Camarillo, the establishment of the company will create employment and improve the infrastructure, therefore leading to an improved economy in the area as noted by Clancy (2000,23).The company, further, has great potential for growth considering it is exploring many other avenues and coping with competition favorably. Reference List Clancy, Kevin. 2000. Counter Intuitive Marketing. London: Free Press. Joshi, Rakesh Mohan. 2005. International Market ing. New Delhi and New York: Oxford University Press. Keller, Kevin Lane. 2002. Strategic Brand Management. London: Prentice Hall. Lenskold, James. 2003. The Path to Campaign, Customer and Corporate Profitability. New York: McGraw-Hill Professional. Masi, Rachel. 1995. Organizational Culture, Distribution And Amount of Control, And Perceptions of Quality. New York: Prentice Hall.Advertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More This essay on Sugar Plum Bakery was written and submitted by user Valentin Rosario to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Monday, November 25, 2019

Free Essays on Ficciones

Uqbar, Tlon, Orbis Tertius Deception is the central idea of this piece. Had the doubtful quote of â€Å"mirrors and copulation are abominable for they multiply the number of mankind† been said by Bioy Casares, the quest to seek the truth of Uqbar would have never existed. This and many other mirror imagery throughout the piece contributes to the deception of reality topic symbolically. â€Å"Mirror troubled the far end of a hallway† because it creates an illusion that the hallway is twice as long as it should be. Deception causes the obscurity of truth- thus the truth that we seek might not be trust worthy. Therefore in attempt to do away with the deception caused by science, the Tlonians â€Å"presuppose idealism† by seeking â€Å"not truth, or even plausibility† only an enlightenment toward an ideally monistic universe. Yet to an even greater degree, the speaker realizes as he describes in the Postscript the creation of Tlon was a deception in itself. The nihilistic Ezra Buckley a ttempts to â€Å"deceive† God by theoretically playing God in the creation of Tlon- Buckley envisions that â€Å"mortals could conceive and shape a world†. Ultimately, the world of Tlon replaces the world as we know it because â€Å"reality ‘caved in’† in the face of the nonexistent deception of truth known as Tlon. Approach to Al-Mu’tasim Borges continues to explore the idea of monism in this piece, but more specifically toward the monism of religion and spirituality. The piece is a â€Å"commentary† of a nonexistent mystery story which explores the religious strife of India- to an extent spiritual and religious strife of the world. The protagonist began his quest after â€Å"he reflects that he has shown himself capable of killing an idolater, yet incapable of knowing†¦whether the Muslim possesses more of truth than the [Hindu] does.† The novel ends with encounter of the protagonist and his quest: Al-Mu’tasim. The ultimate result is the realizat... Free Essays on Ficciones Free Essays on Ficciones Uqbar, Tlon, Orbis Tertius Deception is the central idea of this piece. Had the doubtful quote of â€Å"mirrors and copulation are abominable for they multiply the number of mankind† been said by Bioy Casares, the quest to seek the truth of Uqbar would have never existed. This and many other mirror imagery throughout the piece contributes to the deception of reality topic symbolically. â€Å"Mirror troubled the far end of a hallway† because it creates an illusion that the hallway is twice as long as it should be. Deception causes the obscurity of truth- thus the truth that we seek might not be trust worthy. Therefore in attempt to do away with the deception caused by science, the Tlonians â€Å"presuppose idealism† by seeking â€Å"not truth, or even plausibility† only an enlightenment toward an ideally monistic universe. Yet to an even greater degree, the speaker realizes as he describes in the Postscript the creation of Tlon was a deception in itself. The nihilistic Ezra Buckley a ttempts to â€Å"deceive† God by theoretically playing God in the creation of Tlon- Buckley envisions that â€Å"mortals could conceive and shape a world†. Ultimately, the world of Tlon replaces the world as we know it because â€Å"reality ‘caved in’† in the face of the nonexistent deception of truth known as Tlon. Approach to Al-Mu’tasim Borges continues to explore the idea of monism in this piece, but more specifically toward the monism of religion and spirituality. The piece is a â€Å"commentary† of a nonexistent mystery story which explores the religious strife of India- to an extent spiritual and religious strife of the world. The protagonist began his quest after â€Å"he reflects that he has shown himself capable of killing an idolater, yet incapable of knowing†¦whether the Muslim possesses more of truth than the [Hindu] does.† The novel ends with encounter of the protagonist and his quest: Al-Mu’tasim. The ultimate result is the realizat...

Thursday, November 21, 2019

Critical review of research studies about effective strategies in Lab Report

Critical review of research studies about effective strategies in prolonging breastfeeding - Lab Report Example For mothers’ health, breastfeeding results in reducing risk of breast and ovarian cancers and enhancing maternal infant attachment, more rapid uterine involution, less postpartum depression and weight reduction (Peters, Wehkamp, Felberbaum, Krà ¼ger, & Linder, 2005; USPSTF, 2003). In particular, the decreased risks of breast and ovarian cancers are associated with the duration of breastfeeding in women. Peters et al. (2005) reported that the risk rate is reduced by 4.3% annually among women who breastfeed. Moreover, breastfeeding has cost-benefit. Health Maintenance Organisation in USA (cited in Peters et al., 2005) showed that a family with a child who was breastfed saved $808 for the child in three years. As a result of countless benefits of breastfeeding, the rates of mothers who commence breastfeeding have exponentially grown since 1960s. The World Health Organisation [WHO] (cited in Brttion et al., 2007) strongly recommends that all infants are exclusively breastfed for the first six months of their life. Despite of that, the rate of the breastfeeding continuation is lower than the WHO recommendation. Many infants in developed countries receive breast milk for a shorter period as well as in developing countries (Blyth et al., 2002; Dyson, McCormick, & Renfrew, 2005). In fact, the United Kingdom (UK) and the United States (US) have some of the lowest rates of breastfeeding initiation and duration among developed countries (Blyth et al., 2002; Dyson, McCormick, & Renfrew, 2005). According to WHO, only 35% of infants worldwide receive exclusive breastfeeding in five months postpartum (Peters et al., 2005; Spear, 2004). In Australia, the rate of mothers who continue with breastfeeding drops from 90% to 23% for six months postpartum (Blyth et al., 2002; Cadigan, & Perrin, 2007; Lumbigannon et al., 2007). There are many issues which contribute to short durations of breastfeeding, such as young

Wednesday, November 20, 2019

A scary time to raise a child Research Paper Example | Topics and Well Written Essays - 1250 words

A scary time to raise a child - Research Paper Example While offering nourishment and ensuring that a child does not starve is a major issue when it comes to parenting because it is another mouth to feed in the household, it can be difficult. There are other scary factors to also face when it comes to raise a child which can be deemed as scary. A person may not have time to be a good parent, there is an environment that is intimidating to bring another human into and changing habits in order to raise a child may also be difficult. To raise a child is not as easy as what some people may have expected due to some of the hazards in the world today where there are always situations to fear and worry about. Many times parents do not have time to raise their child. This could be because children are often being raised in single parent homes and there is a lack of people to provide care giving. Parents may be used to being on their own schedule, struggling to make ends meet to pay bills and to afford personal needs for food, shelter and clothing just for themselves. Children are expensive and require a lot of time and nurturing so that they will grow up to become members of society that can grow and prosper. Due to constant demands of the pressure from a present job or from trying to find a job, parents cannot ensure to be fully responsible to their child. They often require daycare or babysitters to help them raise their child until the child is old enough to attend school. As a result of this, they may have less time to pay attention to their childs growth and development which can be detrimental to the child. Most children are also out of school during the summer and while their parents are at work, they are left to be cared for by someone else. As a parent, one has to assume that the babysitter or daycare workers are trustworthy and reliable. The ideal situation is where parents can be flexible so that they can balance work and family responsibilities. Some parents are finding that workplaces

Monday, November 18, 2019

Zara IT for Fast Fashion Essay Example | Topics and Well Written Essays - 2000 words

Zara IT for Fast Fashion - Essay Example The case study "Zara IT for Fast Fashion" indicates that the company relies on an outdated operating system for the store terminals and lacks readily available network across all the stores. The dilemma that the management is grappling with is determining whether they should upgrade the present system and loose the reliability that characterises the current system or proceed with the current DOS-based system that is devoid of compatibility to the future improvements. The case study focuses on Zara, the largest retail chain for Inditex, Spanish retail giant, and the manner in which it has been successful due to its flexibility, excellent fashions and application of a simple business model (McAfee, Dessain and Sjoman, 2007). The head of the IT department in the company, Salgado, is grappling with a dilemma on whether to upgrade the IT capabilities and infrastructure for the retailers or retain the current system. The case study indicates that the company relies on an outdated operating system (OS) for the store terminals and lacks readily available network across all the stores. The Microsoft Disk Operating System (MS-DOS) seems to be working well as no major challenges have been witnessed in the course of its long time use. One of the major concerns expressed by Salgado in the long run is that Zara Point of Sale (POS) is running on MS-DOS that is not supported by Microsoft Inc. According to Capell (2008), DOS has been replaced by Windows since 2001, the same time that Microsoft Inc stopped supporting DOS.

Saturday, November 16, 2019

Cytotoxic Activities and Synthesis of Plumbagin

Cytotoxic Activities and Synthesis of Plumbagin 3.1  Introduction Plants produce a large number of diverse natural products or secondary metabolites which are involved in the normal cell growth, development or reproduction of organisms. Some of these compounds offer protection against herbivores and microbial pathogens, while others are involved in defense against abiotic stress, such as UV-B exposure Many of the secondary metabolites have interesting biological properties, which make them desirable candidates for optimization of drug discovery and development processes. The quinonic moiety is considered by the National Cancer Institute (NCI) as an important biologically scaffold for the development of new bioactive compounds with good levels of cytotoxicity. Clinically important several well-known antitumoral drugs containing a quinone moiety such as anthracyclines, doxorubicin, mitomycin and mitoxantrones and saintopin which are possess a quinonoid structure. These compounds have also been identified as privileged structures due to their biological activity and structural properties that have been linked to the stimulation of oxidative stress and alkylation of cellular nucleophiles in cancer cells. A representative group of quinonoid compounds of naphthoquinones (1,4-naphthoquinones) constitute one of the largest and diverse groups of plant secondary metabolites which are widely distributed in nature with a wide range of important pharmacological activities 3,4 that include antioxidant, antimicrobial, antifeedent, antiinflammatory, anticancer, and allelopathic activity. Some examples of antitumoral naphthoquinones are plumbagin, juglone, b-lapachol and rhinacanthone. Plumbagin (5-hydroxy-2-methyl-1,4-napthoquinone, Fig.la) is a medicinal plant-derived naphthoquinone , which is one of the simplest plant secondary metabolite of three major phylogenic families viz. Plumbaginaceae, Droseraceae, and Ebenceae. Plumbagin is also present in black walnut and other various medicinal plants. It was isolated from the roots of the medicinal plant Plumbag0 zeylanica 1. (also known as Chitrak); The roots of Plumbag0 zeylanica have been used in Indian medicine for more than 2,500 years for treatments of various ailments and which exhibits highly potent biological activities, including antioxidant, antiinflammatory, antibacterial, and antifungal activities. Plumbagin are found in plants as they are thought to be present as a defense mechanism due to their cytotoxicity. It is also used extensively in studies investigating oxidative stress. plumbagin has been shown to exert anticancer and antiproliferative activities in animal models and in cell culture. The mechanism of anti-cancer activity of Plumbagin is reportedly by induction of mammalian topoisomerase II mediated DNA cleavage.4 Plumbagin has been reported to intercalate into the DNA. It induces higher levels of p21 and thereby inhibits long patch base pair excision repairs leading to apoptosis . Hybrid drug molecules of plumbagin by combining plumbagin with other appropriate anticancer agents may lead to the generation of novel and potent anticancer drugs with pleiotropic action against human cancers. 2.2  Present work our group has been engaged in the design and synthesis of more potent, less toxic, and more selective analogues, through chemical modification of lead compounds isolated from the active extracts. In the course of such studies, we have undertaken the plumbago zeylanica, a well-known medicinal plant. The major quantity coupled with the biological profile of plumbagin (1) prompted us to continue our studies. Thus, present work was undertaken to synthesize a library of plumbagin derivatives to study their anti-cancer properties. We here in report the synthesis, cytotoxic activities and their preliminary structure-activity relationship studies. to the best of our knowledge no semi-synthetic derivatives of plumbagin derivaties and test for their till date were reported. (Figure 1). Figure 1: Structure of Plumbagin (1). We focused on the synthesis of new of Plumbagin analogues by following routes. As shown in scheme-1, Piperazines and substituted piperazines are most useful pharmacophores that can be found in many marketed drugs, such as the piperazinyl- linked ciprofloxacin dimmers reported as potent antibacterial agents against resistant strains,15 Merck HIV protease inhibitor Crixivan,16 and drugs under development. A novel class of mixed D2/D4 receptor antagonists, dual calcium antagonist, anti malarial agents and potential antipsychotic agents. Recently piperazine derivatives containing tetrazole nucleus have been reported as an antifungal agent and phytochemists had prepared the derivatives of isolated natural products, which are coupling with piperzenes. This piperzene containing natural products enhance the activity than the parent compound. For designing the analogues of plumbagin a substituted piperzines was introduced at the third position of Plumbagin. This was achieved by the Michael addition of plumbagin with secondary amines and a series of Plumbagin derivatives with the Michael adduct were synthesized. plumbagin (1) was subjected to Michel addition of substituted piperzines with K2C03 in EtoH solvent under roomtemparature conditions for 8 h to yield corresponding substituted piperzine plumbagin analogues (scheme 3.1) in 85 to 95% yield. scheme 3.1 Introduction of propargyl group into hydroxy position of plumbagin scheme 3.2 Preparation of oxime derivatives of plumbagin by substituted acids and substituted piperzines. As shown in scheme 3.3 plumbagin (1) was first subjected to methylation on 0H group of plumbagin and then 9 is treated with NH30H.C1, CH3C0ona in EtoH solvent under room temparature conditions for 6 h to yield corresponding to oxime of 5-0-methyl Plumbagin(lo), it was planned to prepare the new ester derivatives of oxime of 5-0-methyl Plumbagin with various aromatic substituted aromatic carboxylic acids by using standard esterification strategies viz: DDC/DAMP, EDCI/DAMP. Despite the considerable experimentation, however, ester derivatives were never being obtained and moreover, in all cases starting material was completely recovered. In view of these discouraging results, we decided to implement the Yamaguchi esterification protocol (2, 4, 6-trichlorobenz0yl chloride, Et3N, THF, DMAP, toluene) to access ester derivatives. to our delight, the reactions proceeded smoothly to yield the target compounds in moderate to good yields. The structures and yields of all synthesized compounds ( ll21) and the yields were in the range of 80 to 95%. scheme 3.3 oxime of 5-0-methyl Plumbagin(lo) treated with propargyl bromide in presence of K2C03 in acetone solvent under roomtemparature conditions for 8 h to yield corresponding propargyl oxime of 5-0-methyl Plumbagin (22) in 90% yield. According to mannich reaction 22 was treated with secondary amines as substituted piperzines in presence of formaldehyde in ethanol solvent under roomtemparature conditions for lo h to yield corresponding propargyl oximes of 5-0-methyl substituted piperzine plumbagin analogues (23-26) (scheme 3.4) in 85 to 90% yield. All the compounds were synthesized for the first time and well characterized by 1H NMR, 13C NMR and ESI HRMS. biological activity: a). Evaluation of the anti-proliferative activity against Hela, PANC1, MDAMB-231, IMR32, HepG2and SKNSH cell lines: Cancer is one of the most serious threats on human health in the wor1d. The mortality and morbidity of cancer patients is the second highest among all diseases in the wor1d, after heart disease. over the past few decades, extensive research has led to the development of a plethora of chemotherapeutic agents; however, none of these agents are capable of completely eliminating cancer. The limitations of current anticancer drugs, increased incidence and rapid development of drug resistance have highlighted the need for the discovery of new anticancer agents, preferably with novel mechanisms of action. to identify new chemical entities for a more effective treatment of cancer, drug designers can follow many strategies, but the crucial decision is always the selection of a suitable starting point from the vast chemical space . In this respect, natural products can be viewed as evolved privileged structures and biologically pre validated leads, in other words, as molecules that have probably evolved evolutionarily to exert highly specialized functions. Recent review pointed out that, about 74% of anticancer compounds being either natural or natural product-derived products, indicating potency of these scaffolds29. Hence, libraries designed and synthesized around the basic structure of such compounds have better chance of displaying desirable biological and pharmacological properties. As a primary screen for cytotoxic activity, cancer cell growth inhibitory properties of plumbagin derivatives along with parent compound were examined using SKNSH, Hela, HepG2 pancreatic carcinoma cell line (PANC 1), breast cancer cell line (MDA-MB 231), neuroblastoma cell line(IMR-32), by MTT assay. doxorubicin was used as the reference drug and the results are summarized in Table-1. The results revealed that some of the synthetic analogues were exhibited promising anticancer activity when compared their parent isolated compounds. Among the tested all derivatives, compounds 9,12, and 16 showed more potent active against HePG2 cell line with an GI50 value of 0.02 ±0.ol, 0.03 ±0.ol, 0.06 ±0.03  µM respectively and Compounds 3, 5, 24, 25 and 26 manifested potent activity against PANC 1 with an GI50 value of 0.4 ±0.03, 0.ol ±0.ol, 0.3 ±0.03, 0.2 ±0.ol, 0.1 ±0.ol,  µM respectively. While remaining all compounds showed moderate activities on all cell lines. Through it is difficult to discuss the structure activity relationship criteria responsible for the cytotoxic activities in this set of compounds from these results, it can be concluded that me, bis(4-fluorophenyl, 4-ethylpiperazin-1-yl on N in piperezens increase the activity and 0-C1, p-methyl, p-no2 on benzene increase the activity. Table 1: Anti-proliferative activity of plumbagin analogues Table- 3.1: biological activities of Plumbagin analogues (1-26). Sample Hela PANC 1 HepG2 SKNSH MDA-MB-231 IMR32 GI50 GI50 GI50 GI50 GI50 GI50 3.6 ±0.2 2.3 ±0.3 0.7 ±0.1 1.1 ±0.2 8.7 ±0.62 >lo0 14.8 ±0.9 2.9 ±0.7 49.9 ±0.6 4.0 ±0.6 4.3 ±0.65 >lo0 8.4 ±0.7 0.4 ±0.03 >lo0 0.4 ±0.05 2.7 ±0.82 >lo0 2.0 ±0.6 0.9 ±0.6 2.8 ±0.3 0.6 ±0.ol 0.97 ±0.78 0.13 ±0.02 4.1 ±0.9 0.ol ±0.ol >lo0 lo.0 ±0.6 2.3 ±0.7 0.ll ±0.06 4.0 ±3.1 0.5 ±0.07 6.1 ±0.3 3.1 ±0.1 >lo0 >lo0 14.1 ±0.8 2.6 ±1.8 1.7 ±0.2 14.9 ±0.7 0.35 ±0.03 >lo0 19.9 ±1.7 5.5 ±0.4 >lo0 >lo0 0.4 ±0.02 0.17 ±0.08 9.2 ±1.3 6.4 ±0.5 0.02 ±0.ol 1.3 ±0.6 >lo0 >lo0 12.3 ±6.6 0.8 ±0.09 0.5 ±0.06 35.1 ±0.9 6.8 ±0.6 0.046 ±0.ol 6.5 ±1.6 0.9 ±0.06 >lo0 0.9 ±0.09 >lo0 21.5 ±0.5 2.6 ±1.7 1.8 ±0.1 0.03 ±0.ol 1.0 ±0.06 0.79 ±0.07 0.13 ±0.02 3.8 ±1.0 0.4 ±0.1 0.3 ±0.03 0.16 ±0.03 >lo0 0.6 ±0.06 2.3 ±1.2 90.0 ±1.0 12.4 ±1.7 >lo0 5.2 ±0.3 >lo0 16.2 ±0.9 26.4 ±0.9 2.9 ±0.2 4.3 ±0.7 >lo0 >lo0 7.8 ±2.9 24.4 ±6.1 0.06 ±0.03 >lo0 26.2 ±0.63 1.7 ±0.2 8.6 ±1.5 22.2 ±0.7 2.5 ±0.3 >lo0 0.06 >lo0 13.8 ±0.1 25.9 ±0.4 0.2 ±0.04 >lo0 >lo0 >lo0 7.6 ±3.7 18.8 ±1.3 2.0 ±0.9 >lo0 >lo0 0.ll ±0.02 4.1 ±0.3 14.7 ±0.9 0.3 ±0.05 20.5 ±2.2 >lo0 0.34 ±0.07 7.4 ±1.2 16.0 ±1.0 3.5 ±0.4 >lo0 1.8 ±0.6 51.3 ±0.4 5.7 ±0.3 >lo0 34.2 ±2.6 >lo0 1.8 ±0.8 0.32 ±0.05 2.2 ±1.0 0.6 ±0.04 57.0 ±0.7 22.4 ±1.1 5.8 ±0.4 >lo0 1.1 ±0.06 0.3 ±0.03 3.5 ±0.4 >lo0 3.5 ±0.5 >lo0 1.3 ±0.1 0.2 ±0.ol 1.2 ±0.7 12.6 ±3.3 >lo0 19.8 ±0.8 5.2 ±0.4 0.1 ±0.ol 2.0 ±0.2 >lo0 4.7 ±0.78 >lo0 doxorubicin Cytotoxic assy : All cell lines (SIHA, PANC 1, MDA MB -231, IMR -32, DU-145 and A549) used in this study were purchased from the American Type Culture The cell lines (ATCC), United States. The synthesized test compounds were evaluated for their in vitro anti proliferative activity in these six different human cancer cell lines. A protocol of 48 h continuous drug exposure was used, and a SRB cell proliferation assay was used to estimate cell viability or growth. All the cell lines were grown in Dulbecc0s modified Eagles medium (containing lo% FBS in a humidified atmosphere of 5% C02 at 37  °C). Cells were trypsinized when sub-confluent from T25 flasks/60 mm dishes and seeded in 96-well plates in lo0ÃŽ ¼1 aliquots at plating densities depending on the doubling time of individual cell lines. The microliter plates were incubated at 37  °C, 5% C02, 95% air, and lo0% relative humidity for 24 h prior to addition of experimental drugs and were incubated for 48 hrs with different doses (0.ol, 0.1, 1, lo, ,lo0 µM) of prepared derivatives. After 48 hours incubation at 37  °C, cell monolayers were fixed by the addition of lo% (wt/vol) cold trichloroacetic acid and incubated at 4  °C for 1h and were then stained with 0.057% SRB dissolved in 1% acetic acid for 30 min at room temperature. Unbound SRB was washed with 1% acetic acid. The protein –bound dye was dissolved in lomM Tris base solution for 0D determination at 5lo nm using a microplate reader (Enspire, Perkin elmer, USA). Using the seven absorbance measurements [time zero, (Tz), control growth, (C), and test growth in the presence of drug at the five concentration levels (Ti)], the percentage growth was calculated at each of the drug concentrations levels. Percentage growth inhibition was calculated as: [(Ti-Tz)/(C-Tz)] x lo0 for concentrations for which Ti>/=Tz [(Ti-Tz)/Tz] x lo0 for concentrations for which Ti Three dose response parameters were calculated for each experimental agent. Growth inhibition of 50 % (GI50) was calculated from [(Ti-Tz)/(C-Tz)] x lo0 = 50, which is the drug concentration resulting in a 50% reduction in the net protein increase (as measured by SRB staining) in control cells during the drug incubation. The drug concentration resulting in total growth inhibition (TGI) was calculated from Ti = Tz. The 1C50 (concentration of drug resulting in a 50% reduction in the measured protein at the end of the drug treatment as compared to that at the beginning) indicating a net loss of cells following treatment was calculated from [(Ti-Tz)/Tz] x lo0 = -50. Values were calculated for each of these three parameters if the level of activity is reached; however, if the effect is not reached or is exceeded, the value for that parameter was expressed as greater or less than the maximum or minimum concentration tested. 3.4  Conclusion In conclusion, we have successfully synthesized thirty one analogues of Plumbagin (1) and these analogues were evaluated for their anti-cancer activities. Majority of the compounds exhibited significant anti-cancer activity than the parent compound Plumbagin (1) and this study also provides an initial structure–activity data, based on derivatives of Plumbagin (1). The results are an indicative of the fact that the compound 5 proved to be the best analogue with GI50 of 12 proved to be the best analogue with GI50 of invivo studies need to be carried out for revealing the exact mechanism of action and will be taken up in the future in our laboratory. 3.5  Experimental Section Plant material : Plumbag0zeylanica was collected from Tirumala forest, Tirupathi, Andhra Pradesh, India, in August 2007. The plant material was identified by Dr. K. Madhav Chetty, Botany Department, Sri Venkateswara University, Tirupathi. A voucher of the plant was deposited in the Herbarium of the Botany Department. Extaction and isolation: The shade-dried roots of Plumbag0 zeylanica were powdered in a pulvarizer (lo kg) and extracted with chloroform/methanol, 1:1 followed by the concentration under reduced pressure. The resulting extract was (70 g) chromatographed over silica gel (60–120 mesh) and eluted with n-hexane/ethyl acetate combinations of increasing polarity. Plumbagin (12 g) was obtained by elution with n-hexane/ethyl acetate, 99:1. General procedure for the synthesis of piperzene (Secondary amine) derivatives (2-7): to a solution of the Compound 1(leq) in ethanol (3m1) in presence of potassium carbonate as a catalyst was added secondry amine (piperzene) stirred at room temperature for 8 h. The reaction mixture was diluted with Ethyl acetate (lo m1), washed with c0oled ice and brine solution. The organic layer was dried over Na2so4, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography using the mobile phase Acetone in Hexane afforded secondary amine (piperzene) derivatives.

Wednesday, November 13, 2019

Boundaries...What For? :: Argumentative Science Technology Papers

Boundaries...What For? There seems to be no boundaries when it comes to scientific advancement. Scientists are struggling to come up with new and exciting procedures that are supposed to make our lives better. In the process, I think that they are getting out of hand. The advancement of science and technology today has brought up some very interesting and controversial issues. Nowadays, it seems that many scientists are on a mission to create the perfect human being, but in the process are breaking a lot of ethical boundaries. Do scientists know when enough is simply enough?, that is the real question. Through the use of such scientific and technological advancements, its amazing at just what scientists can do with the information. Often times they aren't thinking in terms of the world but in terms of them selves. Which on of them can discover the next biggest thing in science and technology, which can find that cure. It's all a game that is not played fair. There is a lot of craziness going on in the sciences, and with that comes consequences. Take Dr. Frankenstein for instance. In the article Frankenstein by Mary Shelly, she created a character that defied all the scientific and technological boundaries that were around in those days. It was against the law to work with dead bodies, especially for the purposes that he was going to use them for; which was to select the best parts forthe creature he was going to bring to life. He set out to create a "super-human being" out of inatimate objects, and he was successful, but in the end did pay a price. He spent two grueling years that consisted of long days and sleepless nights, for the sole purpose of creating life out of nothing. "For this I deprived myself of rest and health, but now I had finished, the beauty of the dream vanished, and the breathless horror and disgust filled my heart" (Shelly 234). Dr. Frankenstein went way over the line, and tried to play the creator, and in the end got what he deserved, a monster. He abhorred Frankenstein so much, that he chose to alienate him from the world. Which was really unfair to the monster, considering he was created by the sweat of Dr. Frankenstein's brow. Sometimes all that scientific information can get you into trouble. If Dr. Frankenstein had not gone ahead and pursued the role of playing God, he wouldn't have created such an ugly mon ster in his eyes.

Monday, November 11, 2019

Back to the Dream Time

NORTHERN UNIVERSITY OF MALAYSIA COLLEGE OF LAW, GOVERNMENT AND INTERNATIONAL STUDIES SBLE 1043(GROUP D4) TITLE: BACK TO THE DREAMTIME LECTURER: MADAM HAZNUR NADIN BINTI HASSAN PREPARED BY: TEOH BOON KHAI (219673) The two main characters in the novel ‘Back To The Dreamtime’ were Richard and Tom. This novel is talking about how a boy complete the mission that given by his father who had died when he was two years old and how a family maintain their relationship although Richard is adopted by McDonald. Richard Oenpelli McDonald was a smart, rational and filial piety boy.He has a sibling which is Judy and Tom. His mother named Sonya and his father, Oenpelli, tribal name, was died when he was two years old. He was adopted by the Joe McDonalds. However, his parents had hidden him about his background and heritage. Although Richard was Aborigines, he lived in city since he was a kid. He study in University Of Sydney which is same with his brother, Tom. Richard was interesting i n History and he intention to take this course in the university. Tom is Richard’s brother however Tom is a white man and Richard is Aborigines.Tom study in the same university with Richard which is University Of Sydney. Tom was older than Richard exactly two month. Tom was interested in photography and he may continue his study in photography. He found it very interesting and it’s not usual nine to five job. The relationship this two main character was good. They went to school to school together everyday. Besides that, they used to sit and chi-chat beside the railway tracks every morning before going to school. Besides that, Richard, Tom and their sister, Judy were playing cricket in their house backyard.It was their favourite pastime on the weekend. During the break time in school, they and their friend, Bradley used to left the school grounds secretly to go to their favourite place at cliff. Moreover, Richard and Tom decided joined the year’s senior class ca mp which location at Alice Springs with a friend who named Bradley. The year’s camp was taking five days in Alice Springs. Tom volunteer himself to go to Alice Springs with Richard. The purpose they went there is to burn the tjuruga in Richard’s father burial ground at Uluru. At the end, they succeeded to find the burial ground and buried the tjurunga.During the trip, it had drained them physically and emotionally and they experienced it together. They helped each other during the trip. In the conclusion, I feel that the way they maintain their relationship among Richard, Tom and their family is what we have to learn. Although they don’t have any relation in scientific prove, they still very respect and love each other. Nevertheless, they treat their family members very well and take care of each other. They can stay peaceful among each other. This kind of spirit and moral value is what we have to learn from.

Friday, November 8, 2019

Group X essays

Group X essays Middle Eastern Culture and music is much different than American music. Some bands in America try to make groups as a kind of joke towards Middle Easten music. One of those bands in particular is Group X. Group X was formed in October of 1995 by Tom Bestor and Teresa King.Group X was started in the city of Atlanta Georgia. They were hired to play music for a local Heavy Metal and Rock radio station named 99x.The public people of Atlanta liked the new band. Soon the band made a cd that was sold through the radio station and limited stores in Atlanta. The cd was sold out. Almost every copy was purchased. They than decided to make a new cd. It was called "Stepping on the crowtche owf your American Presidaint". They purposely mispelled words to make them sound as if they don't fully know english. The cd was put in almost every music store in Atlanta. They also made a website on the world wide web and sold the cd online.This band ,as new as it is, is still getting more popular every day. The history of Group X is not all so complicated.Tom and Teresa had worked together as freelancers through most of the 1980's. Teresa was a respected producer and Tom a sought-after writer and creative director.Together they worked together on events like national sales conferences for Johnson Although Tom and Teresa enjoyed the lives of freelancers, Tom and Teresa had good ideas on how the creative and production process could be made more effective and efficient. They both felt if they were in charge they would be able to better meat the clients goals. Around the same time one of there clients asked that they form a production entirely of their own. Thus Group X was formed. The company began in Teresa's basement. They were soon forced to move into a larger corporation or building. They moved into a larger building and hired ten employees and still work with 99x as their contract with the radio statio...

Wednesday, November 6, 2019

America First Hand essays

America First Hand essays America First Hand. Robert D. Marcus and David Burner. Beacon Press, 1987, 223 pgs. The beginning of the colonial period was full of troubles. America First Hand, by Robert D. Marcus and David Burner is written in distinct detail about several accounts by American colonists that are written and relay what it was like in the colonies during the first couple decades. The accounts describe how teenagers, men, and women, all in their own way, questioned society, religion, and government in order to improve themselves and have their own effects on a changing county. Marcus and Burner compare and contrast religious, political, and social issues that were dealt with during the early 1800s. The authors examined the experiences of Hetty Shepard, Ben Franklin, and Eliza Pickeny. Three Americans from very different backgrounds who all had thoughts, actions, and ideas to better themselves and hopefully better their fellow man and society. They show that people should try to better themselves based on his or her own opinions and ideas rather than rely on those of others. Miss Hetty Shepard was a Puritan girl living in New England at the height of colonization and had trouble with the Indians. She recorded a diary to describe the days as they went by. Also along with her daily acknowledgements, Hetty reveals private thoughts and inquires that show her doubts and questions concerning her strict Puritan beliefs. Marcus and Burner show passages from a diary written by Hetty Shepard about what some may call the rebellious mind of young woman. Based on her writings, others may call her the beginning of the independent, or republican, women. Men of the colonial time period did not have it much easier than women. Inventor, philosopher, printer, and ladies man extraordinary Ben Franklin was also considered. He was a writer/printer from Philadelphia, and a person who was interested in the science of humanity. Franklin in t...

Monday, November 4, 2019

Religion Essay Example | Topics and Well Written Essays - 250 words - 17

Religion - Essay Example The experience is the troubles that people encounter and eventually abandons their old living habits and follows what their religion teaches them. In the section, â€Å"Theories of religion† (starting from page 8), Flood explains that there are three different schools of thought in how to define religion. Summarize these theories and discuss their strengths and weaknesses. These theories describe religion to be politics in the other way round, religion is a gene and defined religion to be a cultural response to life (Crockett 14). The first schools gave an illustration that based on the aesthetic reduction. The meaning of this is that religion is part of the cultural mechanism that makes sure that the transmission of genes to the other generations is successful. Explanation of how living being come into existence is specified in a range of constraint, this is the advantage of these theories. The limitation for this is that religious resources that pertain human lives in most instances went against the grain of some scholarships. Correct what Flood describes here with your own experience of religion. Does it resonate? Why or not? Basing on what Floods description, it does not resonate with my experience of religion. The reason behind this is that religions are a unifying, purifying, and holy dominion that brings people closer to God (Crockett 15). Floods highlight does not reveal the genesis of man. Instead, he relates with biological aspects that do not exist in the desired religion.

Saturday, November 2, 2019

Illinois Risk Mangement Essay Example | Topics and Well Written Essays - 1750 words

Illinois Risk Mangement - Essay Example This paper has addressed these similarities as well as the differences. In its findings, it was established that the University has a different approach when it comes to the amount of risks it can handle from that of the Illinois government. Overall, one will realize that there are more similarities than differences in these two mainly because they both have people to care for and guarantee both safety and security. The business world is one that is more dependent on a businessperson’s level of knowledge coupled with their ability or willingness to apply it. Because of the unpredictability rife in almost every business, there are several measures taken to either eliminate any risk or mitigate them where they become inevitable. A Risk Management (RM) program is vital for corporate and other institutions to speculate and subsequently plan for the ever-present risks. Often, an institution might still incur losses if the RM program was not properly organized or if there is no clear continuity plan. Because the risks vary with institutions, the RM programs tend to differ. In this paper, a comparison of Illinois and Chicago State University’s RM programs has been done. Chicago State University (CSU) is a learning institution based in Chicago in the state of Illinois that offers several courses to students from all backgrounds. Its major risks include fire, natural calamities, and theft of vital learning equipment and injuries that might occur to students. On the other hand, the government of Illinois (GI) is tasked with providing crucial services to the state’s residents. This onus comes with ensuring that some incidents likely to cause loss of properties or lives are well mitigated. In short, both the CSU and GI have a somewhat similar duty. Both have people to whom security and safety has to be assured. The following section will compare and contrast the two institutions concerning the RM. Worth noting, every RM program foresees risks faced by a firm or